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Polymerase chain reaction assay for the detection of Toxoplasma gondii


Author:
Lunney, J.K., Jauregui, L.H., Higgins, J., Zarlenga, D.S., and Dubey, J.P.
Source:
USDA
Year:
2004
Subject:
sequence analysis, disease detection, patents, ribosomal RNA, DNA primers, polymerase chain reaction, animal health, internal transcribed spacers, toxoplasmosis, mice, human health, USDA, Toxoplasma gondii, and swine
Abstract:
Oligonucleotide primer sequences have been designed which are capable of uniquely amplifying DNA from Toxoplasma gondii when utilized in a polymerase chain reaction assay. In addition, a probe effective for detection of amplification products, particularly in a fluorogenic real time assay system has also been designed. The primers and probe are derived from the internal transcribed spacer 1 (ITS1) region of the T. gondii 18S rRNA gene.
Language:
English
Permanent URL:
http://handle.nal.usda.gov/10113/7063