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Organization and sequence of four flagellin-encoding genes of Edwardsiella ictaluri
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Edwardsiella ictaluri, the cause of enteric septicaemia in channel catfish (Ictalurus punctatus), is motile by means of peritrichous flagella. We determined the complete flagellin gene sequences and their organization in E. ictaluri by sequencing genomic segments from a λ-ZAP phage genomic library of E. ictaluri. Four flagellin genes (fliC1, fliC2, fliC3 and fliC4) are arranged in tandem within 6 kb in the E. ictaluri genome. Each flagellin-coding sequence is preceded by a σ²⁸ recognition site consensus sequence. The predicted amino acid sequences of all four flagellin proteins (between 36 and 37.5 kDa) are similar in the N-terminal (1-160 aa) and C-terminal (last 74 aa) portions and are divergent in the central portion of the proteins. Proteins encoded by flC1, fliC2 and fliC3 are more similar to each other (88-90% aa identity) than to the protein encoded by fliC4 (76-78% aa identity). basic local alignment search tool analysis of GenBank sequences showed that all flagellin aa sequences are more similar to those of Serratia marcescens (72-74% identity) than to those of Edwardsiella tarda ([less-than or equal to]64% identity). Primary determination of E. ictaluri flagellin gene sequences facilitate advanced studies on the role of flagella in host-pathogen interaction.
Panangala, Victor S.
van Santen, Vicky L.
Wolfe, Karen G.
Klesius, Phillip H.
Aquaculture research 2009 July, v. 40, no. 10
Blackwell Publishing Ltd
Journal Articles, USDA Authors, Peer-Reviewed
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
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