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Effects of select nitrocompounds on in vitro ruminal fermentation during conditions of limiting or excess added reductant
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Ruminal methane (CH₄) production results in the loss of up to 12% of gross energy intake and contributes nearly 20% of the United States' annual emission of this greenhouse gas. We report the effects of select nitrocompounds on ruminal fermentation after 22 h in vitro incubation (39°C) with or without additions of hydrogen (H₂), formate or both. In incubations containing no added reductant, CH₄ production was inhibited 41% by 2-nitro-1-propanol (2NPOH) and >97% by 3-nitro-1-propionic acid (3NPA), nitroethane (NE) and 2-nitroethanol (2NEOH) compared to non-treated controls and H₂ did not accumulate. With formate as the sole added reductant, nitro-treatment reduced CH₄ production by >99% and caused 42% to complete inhibition of formate catabolism compared to controls, and the accumulation of H₂ increased slightly. Nitro-treatment decreased CH₄ production 57-98% from that of controls when supplied H₂ or formate plus H₂. Formate catabolism was decreased 42-84% from that in controls by all nitro-treatments except 3NPA with both formate and H₂. Greater than 97% of the added H₂ was catabolized within controls; >84% was catabolized in nitro-treated incubations. Acetate, propionate and butyrate accumulations were unaffected by nitro-treatment irregardless of reductant; however, effects on ammonia and branched chain fatty acid accumulations varied. These results suggest that nitro-treatment inhibited formate dehydrogenase/formate hydrogen lyase and hydrogenase activity.
Anderson, Robin C.
Krueger, Nathan A.
Stanton, Thaddeus B.
Callaway, Todd R.
Edrington, Thomas S.
Harvey, Roger B.
Jung, Yong Soo
Nisbet, David J.
Bioresource technology 2008 Dec., v. 99, no. 18
Journal Articles, USDA Authors, Peer-Reviewed
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