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Flavonoid content and antioxidant capacity of spinach genotypes determined by high-performance liquid chromatography/mass spectrometry

Permanent URL:
http://handle.nal.usda.gov/10113/17185
File:
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Abstract:
BACKGROUND: Flavonoids in different spinach genotypes were separated, identified, and quantified by a high-performance liquid chromatographic method with photodiode array and mass spectrometric detection. The antioxidant capacities of the genotypes were also measured using two antioxidant assays-oxygen radical absorbance capacity (ORACFL) and photochemiluminescence (PCL)-which measure the response to the peroxyl and superoxide anion radicals, respectively.RESULTS: Eighteen flavonoids representing glucuronides and acylated di- and triglycosides of methylated and methylene dioxide derivatives of 6-oxygenated flavonols were identified (patuletin, spinacetin, spinatoside, jaceidin). The total flavonoids ranged from 1805 to 3703 mg kg⁻¹, indicating 2.0-fold variation among genotypes. The ORACFL and PCL values ranged from 48.7 to 84.4 mmol kg⁻¹ and from 9.0 to 14.0 mmol kg⁻¹, respectively, representing as much as 1.7-fold variation among genotypes.CONCLUSION: The ORACFL and PCL values were highly correlated with total flavonoid content (rxy = 0.96).
Author(s):
Cho, Mi Jin , Howard, Luke R. , Prior, Ronald L. , Morelock, Teddy
Note:
Includes references
Source:
Journal of the science of food and agriculture 2008 Apr. 30, v. 88, no. 6
Language:
English
Publisher:
John Wiley & Sons, Ltd.
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.