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1. Alternate intron processing of family 5 endoglucanase transcripts from the genus Phytophthora

Description:
Includes references
Author:
Costanzo, Stefano , Ospina-Giraldo, Manuel D. , Deahl, Kenneth L. , Baker, C. Jacyn , Jones, Richard W.
Subject:
Phytophthora infestans , Phytophthora sojae , Phytophthora ramorum , plant pathogenic fungi , endo-1,4-beta-glucanase , messenger RNA , introns , alternative splicing , genomics , sequence analysis , amino acid sequences , nucleotide sequences
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Year:
2007

2. Diversity of the trifunctional histidine biosynthesis gene (his) in cereal Phaeosphaeria species

Description:
Includes references
Author:
Wang, C.L. , Malkus, A. , Zuzga, S.M. , Chang, P.F.L. , Cunfer, B.M. , Arseniuk, E. , Ueng, P.P.
Subject:
Leptosphaeria nodorum , plant pathogenic fungi , genes , histidine , amino acid metabolism , nucleotide sequences , introns , biotypes , Triticum aestivum , Hordeum vulgare , Secale cereale , Paspalum dilatatum , phylogeny
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Year:
2007

3. Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence

Description:
The avirulence gene AVR-Pita of Magnaporthe oryzae determines the efficacy of the resistance gene Pi-ta in rice. The structures of the AVR-Pita alleles in 39 US isolates of M. oryzae were analyzed using polymerase chain reaction. A series of allele-specific primers were developed from the AVR-Pita gene to examine the presence of AVR-Pita. Orthologous alleles of the AVR-Pita gene were amplified from avirulent isolates. Sequence analysis of five alleles revealed three introns at identical positions in the AVR-Pita gene. All five alleles were predicted to encode metalloprotease proteins highly similar to the AVR-Pita protein. In contrast, the same regions of the AVR-Pita alleles were not amplified in the most virulent isolates, and significant variations of DNA sequence at the AVR-Pita allele were verified by Southern blot analysis. A Pot3 transposon was identified in the DNA region encoding the putative protease motif of the AVR-Pita protein from a field isolate B2 collected from a Pi-ta-containing cultivar Banks. These findings show that transposons can contribute to instability of AVR-Pita and is one molecular mechanism for defeating resistance genes in rice cultivar Banks.
Author:
Zhou, E.X. , Jia, Y.L. , Singh, P. , Correll, J.C. , Lee, F.N.
Subject:
Magnaporthe , plant pathogenic fungi , genes , virulence , alleles , introns , DNA primers , polymerase chain reaction , nucleotide sequences , transposons , pathogenicity , blast disease , Oryza sativa , rice
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Year:
2007

4. Nucleotide sequence analyses of a sugarbeet genomic NPR1-class disease resistance gene

Description:
Includes references
Author:
Kuykendall, L.D. , Murphy, T.S. , Shao, J. , McGrath, J.M.
Subject:
Beta vulgaris , sugar beet , disease resistance , genetic resistance , nucleotide sequences , genes , resistance mechanisms , introns , complementary DNA
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Year:
2007

5. Recent transcontinental sweep of Toxoplasma gondii driven by a single monomorphic chromosome

Description:
Includes references
Author:
Khan, A. , Fux, B. , Su, C. , Dubey, J.P. , Darde, M.L. , Ajioka, J.W. , Rosenthal, B.M. , Sibley, L.D.
Subject:
Toxoplasma gondii , chromosomes , genetic variation , geographical variation , evolution , introns , nucleotide sequences , virulence , toxoplasmosis , mice , disease transmission , population genetics , phylogeny , population structure , adaptive radiation , North America , South America , Europe
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Year:
2007