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Pullulan production by tropical isolates of Aureobasidium pullulans

Permanent URL:
http://handle.nal.usda.gov/10113/976
Abstract:
Tropical isolates of Aureobasidium pullulans previously isolated from distinct habitats in Thailand were characterized for their capacities to produce the valuable polysaccharide, pullulan. A. pullulans strain NRM2, a so-called "color variant" strain, was the best producer, yielding 25.1 g pullulan/l after 7 days in sucrose medium with peptone as the nitrogen source. Pullulan from strain NRM2 was less pigmented than those from the other strains and was remarkably pure after a simple ethanol precipitation. The molecular weight of pullulan from all cultures dramatically decreased after 3 days growth, as analyzed by high performance size exclusion chromatography. Alpha-amylase with apparent activity against pullulan was expressed constitutively in sucrose-grown cultures and induced in starch-grown cultures. When the alpha-amylase inhibitor acarbose was added to the culture medium, pullulan of slightly higher molecular weight was obtained from late cultures, supporting the notion that alpha-amylase plays a role in the reduction of the molecular weight of pullulan during the production phase.
Author(s):
Prasongsuk, S. , Berhow, M.A. , Dunlap, C.A. , Weisleder, D. , Leathers, T.D. , Eveleigh, D.E. , Punnapayak, H.
Subject(s):
Aureobasidium pullulans , carbohydrate metabolism , pullulan , molecular weight , strain differences , enzymatic hydrolysis , alpha-amylase , enzyme inhibitors , strains , tropics , Thailand
Format:
p. 55-61.
Note:
Includes references
Source:
Journal of industrial microbiology & biotechnology 2007 Jan., v. 34, no. 1
Language:
English
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.