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Pullulan production by tropical isolates of Aureobasidium pullulans

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Tropical isolates of Aureobasidium pullulans previously isolated from distinct habitats in Thailand were characterized for their capacities to produce the valuable polysaccharide, pullulan. A. pullulans strain NRM2, a so-called "color variant" strain, was the best producer, yielding 25.1 g pullulan/l after 7 days in sucrose medium with peptone as the nitrogen source. Pullulan from strain NRM2 was less pigmented than those from the other strains and was remarkably pure after a simple ethanol precipitation. The molecular weight of pullulan from all cultures dramatically decreased after 3 days growth, as analyzed by high performance size exclusion chromatography. Alpha-amylase with apparent activity against pullulan was expressed constitutively in sucrose-grown cultures and induced in starch-grown cultures. When the alpha-amylase inhibitor acarbose was added to the culture medium, pullulan of slightly higher molecular weight was obtained from late cultures, supporting the notion that alpha-amylase plays a role in the reduction of the molecular weight of pullulan during the production phase.
Prasongsuk, S. , Berhow, M.A. , Dunlap, C.A. , Weisleder, D. , Leathers, T.D. , Eveleigh, D.E. , Punnapayak, H.
Includes references
Journal of industrial microbiology & biotechnology 2007 Jan., v. 34, no. 1
Journal Articles, USDA Authors, Peer-Reviewed
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