Search National Agricultural Library Digital Collections

NALDC Record Details:

Characterization of a serine carboxypeptidase in the salivary glands and fat body of the orange wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Permanent URL:
http://handle.nal.usda.gov/10113/9406
File:
Download [PDF File]
Abstract:
A full-length cDNA encoding a serine carboxypeptidase (designated SmSCP-1) was recovered from an ongoing salivary gland EST project of the wheat midge. The deduced 461-amino acid sequence had a putative signal sequence at the amino terminus, indicating it was a secreted protein. The protein shared homology with serine carboxypeptidases from other insects, mammals, plants, and yeasts. SmSCP-1 mRNA was expressed in all stages of development and detected in salivary gland and fat body tissues but not in midgut tissue. Expression analysis and quantitative real-time PCR assays in male and female wheat midges and the fat body tissue of adult midges revealed that SmSCP-1 was up-regulated nearly four-fold in the female midges compared to males and nearly two-fold in female fat body compared to male fat body. The wheat midge serine carboxypeptidase (SmSCP-1) most likely has a dual function. As a secreted digestive enzyme, it could play a role in mobilizing host-plant seed reserves for feeding larvae and as expressed in fat body could function as an exopeptidase in degradation of vitellogenin and/or in post-translational processing of other enzymes.
Author(s):
Mittapalli, O. , Wise, I.L. , Shukle, R.H.
Subject(s):
Sitodiplosis mosellana , salivary glands , fat body , serine-type carboxypeptidases , complementary DNA , nucleotide sequences , amino acid sequences , sequence homology , gene expression , messenger RNA , gender differences , protein secretion
Format:
p. 154-160.
Note:
Includes references
Source:
Insect biochemistry and molecular biology 2006 Feb., v. 36, no. 2
Language:
English
Year:
2006
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.