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Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions

Permanent URL:
http://handle.nal.usda.gov/10113/9403
File:
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Abstract:
We have recovered two Hessian fly cytochrome P450 cDNAs from an ongoing midgut EST project. CYP6AZ1 and CYP6BA1 represent two new subfamilies within the CYP6 family. The deduced amino acid sequences for CYP6AZ1 and CYP6BA1 show conserved structural and functional domains of insect P450s. Expression analysis with reverse transcription-polymerase chain reaction (RT-PCR) indicated that CYP6AZ1 is midgut specific and induced during active larval feeding, whereas CYP6BA1 was expressed in all tissues and developmental stages examined. Further expression analysis of CYP6AZ1 with RT-PCR in compatible and incompatible Hessian fly/wheat interactions suggested that CYP6AZ1 may be required for larval feeding in compatible interactions. These results should lead to a better understanding of the Hessian fly/wheat interaction with emphasis on the larval midgut as a critical interface with its host plant.
Author(s):
Mittapalli, O. , Neal, J.J. , Shukle, R.H.
Subject(s):
Mayetiola destructor , cytochrome P-450 , complementary DNA , nucleotide sequences , amino acid sequences , gene expression , messenger RNA , midgut , plant-insect relations , Triticum aestivum , wheat , pest resistance
Format:
p. 981-989.
Note:
Includes references
Source:
Insect biochemistry and molecular biology 2005 Sept., v. 35, no. 9
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.