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Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F
- Abstract::
- The potential biocontrol bacterium Burkholderia ambifaria isolate BC-F significantly suppressed damping-off of cucumber and soybean caused by Pythium ultimum when applied as a seed coating. Cucumber and soybean seed coated with a peat bond formulation of isolate BC-F had significantly greater plant stands than the non-treated pathogen controls in soil-less mix. Soybean seeds treated with isolate BC-F had significantly greater plant fresh weight per pot and plant height per pot than the pathogen control. Isolate BC-F effectively colonized the roots of corn, cucumber, soybean, and sunflower and was detected at greater than or equal to 10(5) colony-forming-units per gram fresh weight of root after 4 weeks in two different natural soils. Burkholderia ambifaria BC-F grown on nutrient broth agar or potato dextrose agar released a diffusable metabolite(s) that inhibited hyphal growth of P. ultimum, Rhizoctonia solani, Phytophthora capsici, and Fusarium oxysporum f.sp. lycopersici. Culture filtrates from isolate BC-F grown on nutrient broth contained a metabolite(s) that inhibited egg hatch and mobility of second-stage juveniles of Meloidogyne incognita. These culture filtrates contained chitinase and protease activities. However, the < 3 kDa fraction of these culture filtrates was responsible for the inhibition of M. incognita. The > 8 kDa fraction contained no inhibitory activity, indicating that inhibition of egg hatch and mobility of second-stage juveniles of M. incognita was not directly due to chitinase and protease activities. The demonstrated ability of isolate BC-F to suppress disease caused by animal and fungal pathogens of many crop plants may be due to the ability of isolate BC-F to persist for long periods in association with roots of diverse crop plants in different soils and the production of a metabolite(s) with broad spectrum anti-biotic activity.
- Author(s):
-
Li, W. , Roberts, D.P. , Dery, P.D. , Meyer, S.L.F. , Lohrke, S. , Lumsden, R.D. , Hebbar, K.P. , Crop protection (Guildford, Surrey)
- Subject(s):
-
bacteria , biological control agents , seed treatment , Pythium ultimum , Thanatephorus cucumeris , Phytophthora capsici , Fusarium oxysporum f. sp. lycopersici , Meloidogyne incognita , plant pathogenic fungi , plant parasitic nematodes , roots , bioassays , crops , nematode control , biological control
- Description:
- Includes references
- Source:
- Crop protection Mar 2002. v. 21 (2)
- Language:
- English
- Year:
- 2002
- Collection:
-
Journal Articles, USDA Authors, Peer-Reviewed
- File:
-
Download [PDF]
- Rights:
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
