Search National Agricultural Library Digital Collections

NALDC Record Details:

Selection of Heterodera glycines chorismate mutase-1 alleles on nematode-resistant soybean

Permanent URL:
http://handle.nal.usda.gov/10113/7308
File:
Download [PDF File]
Abstract:
The soybean cyst nematode Heterodera glycines is the most destructive pathogen of soybean in the Unites States. Diversity in the parasitic ability of the nematode allows it to reproduce on nematode-resistant soybean. H. glycines chorismate mutase-1 (Hg-CM-1) is a nematode enzyme with the potential to suppress host plant defense compounds; therefore, it has the potential to enhance the parasitic ability of nematodes expressing the gene. Hg-cm-1 is a member of a gene family where two alleles, Hg-cm-1A and Hg-cm-1B, have been identified. Analysis of the Hg-cm-1 gene copy number revealed that there are multiple copies of Hg-cm-1 alleles in the H. glycines genome. H. glycines inbred lines were crossed to ultimately generate three F2 populations of second-stage juveniles (J2s) segregating for Hg-cm-1A and Hg-cm-1B. Segregation of Hg-cm-1A and 1B approximated a 1:2:1 ratio, which suggested that Hg-cm-1 is organized in a cluster of genes that segregate roughly as a single locus. The F2 H. glycines J2 populations were used to infect nematode-resistant (Hartwig, PI88788, and PI90763) and susceptible (Lee 74) soybean plants. H. glycines grown on Hartwig, Lee 74, and PI90763 showed allelic frequencies similar to Hg-cm-1A/B, but nematodes grown on PI88788 contained predominately Hg-cm-1A allele as a result of a statistically significant drop of Hg-cm-1B in the population. This result suggests that specific Hg-cm-1 alleles, or a closely linked gene, may aid H. glycines in adapting to particular soybean hosts.
Author(s):
Lambert, K.N. , Bekal, S. , Domier, L.L. , Niblack, T.L. , Noel, G.R. , Smyth, C.A.
Subject(s):
alleles , Heterodera glycines , Glycine max , soybeans , host-parasite relationships , pest resistance , cyst nematodes , isomerases , inbred lines , animal genetics , nematode biology , infection , loci
Format:
p. 593-601.
Note:
Includes references
Source:
Molecular plant-microbe interactions : MPMI 2005 June, v. 18, no. 6
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.