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Allelic variation and distribution independence of Puroindoline b-B2 variants and their association with grain texture in wheat

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In this study, we identify the allelic variation of the Pinb-B2v3 variant, which could be divided into three different alleles, Pinb-B2v3a, Pinb-B2v3b and Pinb-B2v3c. The result of v2 tests showed that the distribution of Puroindoline b-2 variants has different frequencies in common and durum wheats. Analysis of the association of Pinb-B2v with grain hardness indicated that wheat cultivars with Pinb-B2v3b possessed relatively higher single kernel characterization system (SKCS) hardness indices in soft wheat in the 2006–2007 cropping season. Further analysis of SKCS hardness among different Puroindoline B-b2 variants by an F8 recombinant inbred line (RIL) population containing 350 RILs indicated that lines with Pinb-2v3b were on average 5.4 SKCS hardness index units harder than those carrying the Pinb-2v2 haplotype. Derived cleaved amplified polymorphic sequence markers were developed for identification of Pinb-B2v3b and Pinb-B2v3c alleles and will be useful for screening early generation materials by marker-assisted selection during wheat breeding. The results of quantitative real-time PCR indicated that the relative expression level of Pinb-B2v3b was significantly higher than those of Pinb-B2v2, Pinb-B2v3a and Pinb-B2v3c, that four Pinb-B2 alleles showed the highest relative expression level on the 14th day after anthesis during grain development, and that relative expression levels of Pinb-B2v3b and Pinb-B2v2 in leaf were significantly higher than those in root, suggesting that PINB-2 are possibly not seedspecific proteins and that the expression level of Pinb-B2v3 was possibly positively correlated with grain hardness.
Feng Chen , Fuyan Zhang , Huanhuan Li , Craig F. Morris , Yingying Cao , Xiaoli Shang , Dangqun Cui
alleles , correlation , cultivars , haplotypes , hardness , inbred lines , leaves , marker-assisted selection , proteins , quantitative polymerase chain reaction , screening , seed development , seeds , texture , wheat
USDA Scientist Submission
Molecular breeding 2013 v.32
Journal Articles, USDA Authors, Peer-Reviewed
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