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Construction of black (Rubus occidentalis) and red (R. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach

Permanent URL:
http://handle.nal.usda.gov/10113/58546
File:
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Abstract:
The genus Rubus belongs to the Rosaceae and is comprised of 600-800 species distributed worldwide. To date, genetic maps of the genus consist largely of non-transferable markers such as amplified fragment length polymorphisms (AFLP). An F1 population developed from a cross between an advanced breeding selection of Rubus occidentalis (96395S1) and R. idaeus ‘Latham’ was used to construct a new genetic map consisting of DNA sequence-based markers. The genetic linkage maps presented here are constructed of 131 markers on at least one of the two parental maps. The majority of the markers are orthologous, including 14 Rosaceae conserved orthologous set (RosCOS) markers, and 60 new gene-based markers developed for raspberry. Thirty-four published raspberry simple sequence repeat (SSR) markers were used to align the new maps to published raspberry maps. The 96395S1 genetic map consists of six linkage groups (LG) and covers 309 cM with an average of 10 cM between markers; the ‘Latham’ genetic map consists of seven LG and covers 561 cM with an average of 5 cM between markers. The use of orthologous genetic markers allows this raspberry genetic linkage map to be compared with the sequenced genomes of other Rosaceae members such as strawberry, apple and peach. Our results suggest a high degree of synteny between the raspberry and strawberry genomes and gives unprecedented insights into the genome evolution of raspberry from the putative ancestral genome of the single ancestor common to Rosaceae.
Author(s):
J. M. Bushakra , M. J. Stephens , A. N. Atmadjaja , K. S. Lewers , V. V. Symonds , J. A. Udall , D. Chagné , E. J. Buck , S. E. Gardiner
Subject(s):
DNA , Fragaria vesca , Malus , Prunus , Rubus occidentalis , amplified fragment length polymorphism , apples , chromosome mapping , evolution , genome , linkage groups , nucleotide sequences , peaches , strawberries
Source:
Theoretical and applied genetics 2012
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.