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Utilizing Soybean Milk to Culture Soybean Pathogens

Permanent URL:
http://handle.nal.usda.gov/10113/58459
File:
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Abstract:
Liquid and semi-solid culture media are used to maintain and proliferate bacteria, fungi, and Oomycetes for research in microbiology and plant pathology. In this study, a comparison was made between soybean milk medium, also referred to as soymilk, and media traditionally used for culturing soybean pathogens to determine if soymilk medium was an effective medium for growth of Colletotrichum truncatum, Fusarium virguliforme, Macrophomina phaseolina, Passalora sojina, Phomopsis longicolla, Phytophthora sojae, Pythium irregulare, Rhizoctonia solani, and Sclerotinia sclerotiorum. Based on radial mycelial colony growth rates, C. sojina grew significantly (P < 0.05) faster on soymilk dextrose agar (SDA) than on V-8 agar, and C. truncatum and F. virguliforme grew significantly (P < 0.05) faster on SDA than potato dextrose agar (PDA). Significantly (P < 0.001) greater masses of sclerotia were produced by S. sclerotiorum grown on SDA as compared to PDA. Soymilk used with agar or used alone as a broth may be an option for replacing more expensive processed culture media.
Author(s):
Yiwen Xiang , Theresa Herman , Glen L. Hartman
Subject(s):
Colletotrichum truncatum , Fusarium , Macrophomina phaseolina , Phomopsis longicolla , Phytophthora sojae , Pythium irregulare , Sclerotinia sclerotiorum , Thanatephorus cucumeris , agar , bacteria , culture media , fungi , glucose , microbial growth , pathogens , plant pathology , sclerotia , soybeans , soymilk
Source:
Advances in Microbiology 2014 1 v.4
Language:
English
Year:
2014
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.