Search National Agricultural Library Digital Collections

NALDC Record Details:

Apolipoprotein A1 in channel catfish: Transcriptional analysis, antimicrobial activity, and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

Permanent URL:
http://handle.nal.usda.gov/10113/58420
File:
Download [PDF File]
Abstract:
The objectives of this study were to: 1) determine transcriptional profiles of apolipoprotein A1 (ApoA1) in collected channel catfish tissues after infection with Aeromonas hydrophila by bath immersion; 2) investigate whether recombinant channel catfish apolipoprotein A1 produced in Escherichia coli expression system possesses any antimicrobial activity against A. hydrophila; 3) evaluate whether recombinant channel catfish apolipoprotein A1 plasmid DNA could be used as immunostimulant to protect fish against A. hydrophila infection. Quantitative PCR revealed that the transcription levels of ApoA1 in infected catfish were significantly (P < 0.05) more induced in the anterior kidney. Recombinant apoA1 produced in E. coli expression system exhibited lytic activity against Gram-positive Micrococcus lysodeikticus and Gram-negative A. hydrophila. When pcDNA3.2-vectored recombinant apoA1 was transfected in channel catfish gill cells G1B, the over-expression of pcDNA-ApoA1 offered significant (P < 0.05) protection to G1B cells against A. hydrophila infection. When channel catfish were intraperitoneally injected with QCDCR adjuvant formulated pcDNA-ApoA1 and challenged with a highly virulent A. hydrophila strain AL-09-71 at two days post injection, pcDNA-ApoA1 injection offered 100% protection to channel catfish. Macrophages of fish injected with pcDNA-ApoA1 produced significantly (P < 0.05) higher amounts of reactive oxygen species and nitric oxide than that of fish injected with pcDNA vector alone. Our results suggest that pcDNA-ApoA1 could be used as a novel immunostimulant to offer immediate protection to catfish against A. hydrophila infection.
Author(s):
Julia W. Pridgeon , Phillip H. Klesius
Subject(s):
Aeromonas hydrophila , Escherichia coli , Ictalurus punctatus , Micrococcus luteus , antimicrobial properties , apolipoprotein A-I , bacterial infections , catfish , fish diseases , gene overexpression , gills , immunization , kidneys , macrophages , nitric oxide , plasmids , polymerase chain reaction , reactive oxygen species , recombinant proteins , transcription (genetics) , transfection
Source:
Fish and Shellfish Immunology 2013 v.35
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.