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A functional genomic analysis of Arabidopsis thaliana PP2C clade D

Permanent URL:
http://handle.nal.usda.gov/10113/58416
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Abstract:
In the reference dicot plant Arabidopsis thaliana, the PP2C family of P-protein phosphatases includes the products of 80 genes that have been separated into 10 multi-protein clades plus six singletons. Clade D includes the products of nine genes distributed among 3 chromosomes (PPD1, At3g12620; PPD2, At3g17090; PPD3, At3g51370; PPD4, At3g55050; PPD5, At4g33920; PPD6, At4g38520; PPD7, At5g02760; PPD8, At5g06750; and PPD9, At5g66080).As part of a functional genomics analysis of protein phosphorylation, we retrieved expression data from public databases and determined subcellular protein localization of the members of Clade D. While the nine proteins have been grouped together based upon primary sequence alignments, we observed no obvious common patterns in expression or localization. We found chimera with the GFP associated with the nucleus, plasma membrane, the endomembrane system, and mitochondria in transgenic plants.
Author(s):
Alejandro Tovar-Mendez , Jan A. Miernyk , Elizabeth Hoyos , Douglas D. Randall
Subject(s):
Arabidopsis thaliana , chromosomes , databases , endomembrane system , genes , genomics , mitochondria , phosphoprotein phosphatase , plasma membrane , protein phosphorylation , proteins , transgenic plants
Source:
Protoplasma 2014 v.251
Language:
English
Year:
2014
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.