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DNA barcoding to identify all life stages of holocyclic cereal aphids (Hemiptera: Aphididae) on wheat and other poaceae

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The cytochrome c oxidase subunit I (COI) gene of the mtDNA was sequenced in eight holocyclic monoecious aphids that occur on wheat, barley, oat, and sorghum in the US. The first 640 bp of the 3' end were considered as a DNA barcoding technique for species identification. DNA barcoding successfully differentiated Schizaphis graminum (Rondani), Diruaphis noxia (Kurdjumov), D. tritici (Gillette), D. frequens (Walker), D. mexicana (McVicar Baker), Sipha flava (Forbes), S. elegans del Guercio, and Sitobion avenae (F.). In addition to the above monoecious species, the common cereal aphids Rhopalosiphum padi (L.) and R. maidis (Fitch) were also included and successfully differentiated. DNA barcoding is a reliable alternative to traditional morphology in the identification of cereal aphids and their various life stages and morphs, including eggs. Its use will be able to confirm whether D. noxia is now reproducing sexually in the US after 20 years of asexual reproduction.
Kevin A Shufran , Gary J Puterka
Avena sativa , DNA barcoding , Diuraphis noxia , Hemiptera , Hordeum vulgare , Rhopalosiphum maidis , Rhopalosiphum padi , Schizaphis graminum , Sipha flava , Sitobion avenae , Sorghum bicolor , Triticum aestivum , asexual reproduction , barley , cytochrome-c oxidase , mitochondrial DNA , oats , taxonomy , wheat , United States
Annals of the entomological society of america 2011 2 1 v.104 no.1
Journal Articles, USDA Authors, Peer-Reviewed
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