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A stem-loop structure in the 5' untranslated region of bean pod mottle virus RNA2 is specifically required for RNA2 accumulation

Permanent URL:
http://handle.nal.usda.gov/10113/58210
Abstract:
Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus of the family Secoviridae. Its RNA1 encodes all proteins needed for genome replication and is capable of autonomous replication. By contrast, BPMV RNA2 must utilize RNA1-encoded proteins for replication. Here, we sought to identify RNA elements in RNA2 required for its replication. The exchange of 59 untranslated regions (UTRs) between genome segments revealed an RNA2-specific element in its 59 UTR. Further mapping localized a 66 nucleotide region that was predicted to fold into an RNA stem–loop structure, designated SLC. Additional functional analysis indicated the importance of the middle portion of the stem and an adjacent two-base mismatch. This is the first report of a cis-acting RNA element in RNA2 of a bipartite secovirus.
Author(s):
Junyan Lin , Ahmed Khamis Ali , Ping Chen , Said Ghabrial , John Finer , Anne Dorrance , Peg Redinbaugh , Feng Qu
Subject(s):
5' untranslated regions , Bean pod mottle virus , genome , molecular conformation , plant viruses , proteins
Source:
Journal of General Virology 2013 6 1 v.94
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.