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Ribotype and serotype diversity of Escherichia coli isolates from poultry and human sources

Permanent URL:
http://handle.nal.usda.gov/10113/58176
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Abstract:
Escherichia coli isolates from the Universidad Nacional Autónoma de México were analyzed to evaluate the genetic relatedness and serotype. Ribotype was determined using the RiboPrinter® Microbial Characterization System following DNA digestion with the restriction enzyme EcoRI. A total of 41 ribogroups were isolated. These segregated into three clusters, one cluster contained only poultry isolates, the second contained only human isolates and the third contained 55% poultry isolates and 45% human isolates. Results showed a high level of ribotypic relatedness within the E. coli isolates that could infect both poultry and human sources. E. coli isolates collected from exclusively poultry or human sources showed a high level of ribotypic diversity. Serotype was determined using antisera against somatic (O) antigens. A total of 22 serotypes were identified, of which six were typical of the avian pathogenic E. coli (APEC) strains and six contained both human and poultry isolates.
Author(s):
Cynthia Sheffield , Cecilia Rosario-Cortés , Kenneth Bischoff
Subject(s):
DNA , Escherichia coli , animal pathogenic bacteria , antiserum , biodiversity , birds , cluster analysis , digestion , genetic relationships , genetic variation , humans , poultry , restriction endonucleases , restriction fragment length polymorphism , ribotypes , serotypes , somatic antigens , Mexico
Source:
International Journal of Food 2010 12 22 v.8 no.3/4
Language:
English
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.