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Differentiating among plant spectra by combining pH dependent photoluminescence spectroscopy with multi-way principal component analysis (MPCA)

Permanent URL:
http://handle.nal.usda.gov/10113/58154
File:
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Abstract:
Photoluminescence spectroscopic probes offer the potential for real-time measurements of animal diet composition. Spectral emission signatures (excitation at 365 nm) from three different pH (2.2, 7.5 and 12.5) phosphate buffered saline (PBS) extracts from two grasses, Sporobolus flexuosus (Thurb. ex Vasey) Rydb., [mesa dropseed], and Pleuraphis mutica Buckley [tobosa], two forbs, Dimorphocarpa wislizenii (Engelm.) Rollins [spectacle pod], and Sphaeralcea incana Torrey [pale globemallow], and leaves and twigs from two shrubs Flourensia cernua DC. [tarbush], and Atriplex canescens (Pursh) Nutt., [fourwing saltbush] were examined. Since pH has been shown to be pivotal in affecting extraction efficiency of other plant compounds pH seemed appropriate as an additional dimension within our multi-way principal component analysis (MPCA) to differentiate among six different plant species. In particular, MPCA allowed differentiation between Sporobolus and Pleuraphis that was not possible using only principal component analysis (PCA). This research suggests MPCA may be a more appropriate tool than PCA when attempting to discriminate among plant species.
Author(s):
Dean M. Anderson , Timothy L. Danielson , Safwan M. Obeidat , Gary D. Rayson , Rick E. Estell , Baolong Bai , Ed L. Fredrickson
Subject(s):
Atriplex canescens , Flourensia , Sphaeralcea , Sporobolus , animals , branches , diet , forbs , grasses , leaves , pH , principal component analysis , shrubs , spectroscopy
Source:
The Open Agriculture Journal 2011 v.5
Language:
English
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.