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Development of resistance to eCry3.1Ab-expressing transgenic maize in a laboratory-selected population of western corn rootworm (Coleoptera: Chrysomelidae)

Permanent URL:
http://handle.nal.usda.gov/10113/58148
Abstract:
There was a significant colony × maize pedigree interaction in terms of the number of larvae recovered. There was no significant difference in the number of larvae recovered from eCry3.1Ab-expressing and isoline maize for the selected colony, whereas this difference was significant for the control colony. There was not a significant colony × maize pedigree interaction in terms of root damage, or the number of beetles recovered, but the effect of maize pedigree was significant. After four and eight generations of selection, seedling bioassays were performed. Again, there was a significant colony × maize pedigree interaction in terms of the number of larvae recovered. Larvae of the selected colony, and offspring of reciprocal crosses of the control and selected colonies, had higher LC50 values than the control colony when exposed to increasing concentrations of the eCry3.1ab protein. Resistance ratios of the selected colony, selected female offspring colony, and selected male offspring colony were 2.58, 3.34, and 7.58, respectively. These data provide necessary information for understanding the potential for Bt resistance by western corn rootworm and will be useful for developing improved insect resistance management plans.
Author(s):
Daniel L. Frank , Anthony Zukoff , Julie Barry , Matthew L. Higdon , Bruce E. Hibbard
Subject(s):
Bacillus thuringiensis , Diabrotica virgifera virgifera , artificial selection , bacterial insecticides , bioassays , corn , crop damage , defense mechanisms , females , host-parasite relationships , insect colonies , insecticidal proteins , insects , isogenic lines , larvae , lethal concentration 50 , males , pedigree , population , progeny , reciprocal crosses , resistance management , seedlings , survival rate , transgenic plants
Source:
Journal of economic entomology 2013 v.106 no.6
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.