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Development of resistance to eCry3.1Ab-expressing transgenic maize in a laboratory-selected population of western corn rootworm (Coleoptera: Chrysomelidae)

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There was a significant colony × maize pedigree interaction in terms of the number of larvae recovered. There was no significant difference in the number of larvae recovered from eCry3.1Ab-expressing and isoline maize for the selected colony, whereas this difference was significant for the control colony. There was not a significant colony × maize pedigree interaction in terms of root damage, or the number of beetles recovered, but the effect of maize pedigree was significant. After four and eight generations of selection, seedling bioassays were performed. Again, there was a significant colony × maize pedigree interaction in terms of the number of larvae recovered. Larvae of the selected colony, and offspring of reciprocal crosses of the control and selected colonies, had higher LC50 values than the control colony when exposed to increasing concentrations of the eCry3.1ab protein. Resistance ratios of the selected colony, selected female offspring colony, and selected male offspring colony were 2.58, 3.34, and 7.58, respectively. These data provide necessary information for understanding the potential for Bt resistance by western corn rootworm and will be useful for developing improved insect resistance management plans.
Daniel L. Frank , Anthony Zukoff , Julie Barry , Matthew L. Higdon , Bruce E. Hibbard
Bacillus thuringiensis , Diabrotica virgifera virgifera , artificial selection , bacterial insecticides , bioassays , corn , crop damage , defense mechanisms , females , host-parasite relationships , insect colonies , insecticidal proteins , insects , isogenic lines , larvae , lethal concentration 50 , males , pedigree , population , progeny , reciprocal crosses , resistance management , seedlings , survival rate , transgenic plants
Journal of economic entomology 2013 v.106 no.6
Journal Articles, USDA Authors, Peer-Reviewed
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