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Destruction of single-species biofilms of Escherichia coli or Klebsiella pneumoniae subsp. pneumoniae by dextranase, lactoferrin, and lysozyme

Permanent URL:
http://handle.nal.usda.gov/10113/58143
File:
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Abstract:
The aim of this work was to determine the destructive activity of dextranase, lactoferrin, and lysozyme, against single species biofilms composed of either Klebsiella pneumoniae subsp. pneumoniae or Escherichia coli using the MBEC Assay. Luminescence measurements based on quantitation of the ATP present were used to determine the amount of biofilm elimination and correlated with quantity of live bacteria present in the sample. The data were analyzed employing a two-way ANOVA and Bonferroni post-test. Treatments resulted in percentage reductions of E. coli biofilms ranging from 73 to 98 %. Lactoferrin (40 μg/ml) produced a signifiantly higher-percentage reduction than lysozyme (10 μg/ml) (P < 0.05), no other significant differences occurred. Similar treatments resulted in percentage reductions of K. pneumoniae subsp. pneumoniae biofilms ranging from 51 to 100 %. Dextranase treatments produced a significantly lower percentage reduction than all other materials (P < 0.05), no other significant differences occurred. No material was capable of complete destruction of both single species biofilms; however, low concentrations of lactoferrin and lysozyme each removed 100 % of the K. pneumoniae subsp. pneumoniae biofilm. Low concentrations of lactoferrin or lysozyme might be beneficial to prevent biofilm formation by K. pneumoniae subsp. pneumoniae.
Author(s):
Cynthia L. Sheffield , Tawni L. Crippen , Toni L. Poole , Ross C. Beier
Subject(s):
Escherichia coli , Klebsiella pneumoniae subsp. pneumoniae , adenosine triphosphate , analysis of variance , antibacterial proteins , antimicrobial agents , bacteria , bioassays , biofilm , data analysis , dextranase , lactoferrin , luminescence , lysozyme
Source:
International Microbiology 2012 12 v.15 no.4
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.