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Chicken-specific peptide arrays for kinome analysis: Flight for the flightless

Permanent URL:
http://handle.nal.usda.gov/10113/58138
File:
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Abstract:
Kinomics, the study of kinase enzymes within an organism, is a rapidly growing field of proteomics. The use of high-throughput technology to study the kinome has enabled researchers to conduct studies of the global signaling environment within an organism. The problem arises when researchers interested in non-human, non-mouse species attempt to use these latest techniques for their species of interest. A recent advancement that has overcome this species problem is the species-specific peptide array. Custom tailored to the species of interest, this high-throughput kinome technology allows researchers to study global cellular signaling events in nearly any organism that uses phosphorylation-mediated signal transduction. Specific to this review is the study of the chicken, which has never been a more important or relevant research species. There are a number of basic biological questions about chickens that can be answered through new experimental techniques. In addition, zoonotic diseases, like avian influenza and Salmonella, which can infect humans through interaction with infected animals, have shown avians to be an important infectious vector. While the significant limitations to the mouse model have become more and more apparent, researchers have turned to alternative species such as chicken which are relatively easy to care for, inexpensive, and are suited to large scale studies. The chicken is an ideal candidate for in ovo developmental studies, as well as models for certain infectious agents. Finally, issues of food safety and agricultural antibiotic use are ever present in the media and public policy discussions. The development of research tools to find safer means of animal production and alternatives to antibiotics are going to be increasingly important research objectives in the years to come.
Author(s):
Ryan J. Arsenault , Michael H. Kogut
Subject(s):
Salmonella , agricultural health and safety , animal models , animal production , antibiotics , avian influenza , chickens , disease vectors , eggs , embryology , food safety , humans , kinases , pathogens , peptides , phosphorylation , proteome , proteomics , public policy , research and development , researchers , signal transduction , zoonoses
Source:
Current Topics in Biotechnology 2012 v.7
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.