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SnTox5-Snn5: A novel Stagonospora nodorum effector-wheat gene interaction and its relationship with the SnToxA-Tsn1 and SnTox3-Snn3-B1 interactions.

Permanent URL:
http://handle.nal.usda.gov/10113/58119
File:
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Abstract:
The Stagonospora nodorum-wheat interaction consists of multiple pathogen - produced necrotrophic effectors that interact directly or indirectly with specific host gene products to induce the disease Stagonospora nodorum blotch (SNB). Here, we used a tetraploid wheat mapping population to identify and characterize a sixth effector-host gene interaction in the wheat-S. nodorum system. Initial characterization of the effector, designated SnTox5, indicated that it is a proteinaceous necrotrophic effector that induces necrosis on host lines harboring the Snn5 sensitivity gene, which we mapped to the long arm of wheat chromosome 4B. Based on ultra-filtration, SnTox5 is likely in the size range of 10 to 30 kDa. Analysis of SNB development in the mapping population indicated that the SnTox5-Snn5 interaction explained from 37 to 63% of the variation, demonstrating that this interaction plays a significant role in disease development. The effects of a compatible SnTox5-Snn5 interaction were additive with those of a compatible SnToxA-Tsn1 interaction, and when the two interactions occurred together the level of SNB was significantly increased. However, the effects of SnTox5-Snn5 were epistatic to the SnTox3-Snn3-B1 interaction. Similar to several other interactions in this system, the SnTox5-Snn5 interaction is light dependent suggesting that multiple interactions may exploit the same pathways to cause disease.
Author(s):
Timothy L. Friesen , Chenggen Chu , Steven S. Xu , Justin D. Faris
Subject(s):
Leptosphaeria nodorum , Triticum aestivum , chromosome mapping , chromosomes , epistasis , fungal diseases of plants , genes , host-pathogen relationships , mycotoxins , necrosis , pathogens , plant proteins , tetraploidy , wheat
Source:
Molecular plant pathology 2012 v.13 no.9
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.