Search National Agricultural Library Digital Collections

NALDC Record Details:

Developing tools for investrigating the multiple roles of ethylene: Identification and mapping genes for ethylene biosynthesis and reception in barley.

Permanent URL:
Download [PDF File]
The plant hormone ethylene is important to many plant processes from germination through senescence, including responses to in vitro growth and plant regeneration. Knowledge of the number of genes, and of their function, that are involved in ethylene biosynthesis and reception is necessary to determine the role of specific genes within gene families known to influence ethylene biosynthesis and other aspects of ethylene function in plants. Our objective was built on previous studies that have established the critical role of ethylene in the in vitro response of barley (Hordeum vulgare L.), and that have identified ethylene-related QTLs in the barley genome. In this study, we have identified multiple members, and their locations, of genes in the barley 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS), ACC oxidase (ACO), and ethylene receptor (ETR) gene families. Specific primers for PCR amplification of each gene were developed and used for mapping in the Oregon Wolf Barley mapping population. Five ACS, 8 ACO, and 7 ETR genes were identified and mapped to six of the barley chromosomes. Gene locations were syntenous to the orthologs in rice except for two that mapped to chromosome 6H. Gene duplication was evident for ACO genes on chromosomes 5H and 6H. Gene-specific primers will be useful for determining expression of each gene under various environmental conditions, including in vitro environments, to better understand the role of ethylene.
Lynn S. Dahleen , Neerja Tyagi , Phil Bregitzer , Ryan H. Brown , William C. Morgan
1-aminocyclopropane-1-carboxylate synthase , 1-aminocyclopropane-1-carboxylic acid , DNA primers , Hordeum vulgare , aminocyclopropanecarboxylate oxidase , barley , chromosome mapping , chromosomes , environmental factors , ethylene , ethylene production , gene duplication , genes , plant growth , polymerase chain reaction , quantitative trait loci , receptors , rice , seed germination
Molecular genetics and genomics 2012 v.287
Journal Articles, USDA Authors, Peer-Reviewed
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.