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Synergistic effects of green tea polyphenols and alphacalcidol on chronic inflammation-induced bone loss in female rats

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Summary: Studies suggest that green tea polyphenols (GTP) or alphacalcidol is promising agent for preventing bone loss. Findings that GTP supplementation in the drinking water plus alphacalcidol administration resulted in increased bone mass via a decrease of oxidative stress and inflammation suggest a significant role of GTP plus alphacalcidol in bone health of women with chronic inflammation. Introduction: Recent studies have suggested that green tea polyphenols (GTP) or alphacalcidol are promising dietary supplements for preventing bone loss in women. However, the mechanism(s) related to the possible synergistic osteo-protective role of GTP plus D3 in bone loss due to chronic inflammation is not well understood. Methods: This study evaluated bioavailability, efficacy, and related molecular mechanisms of GTP in combination with alphacalcidol in conserving bone loss in rats with chronic inflammation. A 12-week study of 2 (no GTP vs. 0.5% GTP in drinking water) × 2 (no alphacalcidol vs. 0.05 µg/kg alphacalcidol, 5×/week) factorial design in lipopolysaccharide17 administered female rats was performed. Additionally, a group receiving placebo administration was used to compare with a group receiving lipopolysaccharide administration only to evaluate the effect of lipopolysaccharide. Results: Lipopolysaccharide administration resulted in lower values for bone mineral content and density, but higher values for serum tartrate resistant acid phosphatase (TRAP), urinary 8-hydroxy-2’-deoxyguanosine, and mRNA expression of tumor necrosis factor-a and cyclooxygenase-2 in spleen. GTP supplementation increased urinary epigallocatechin and epicatechin concentrations. Both GTP supplementation and alphacalcidol administration resulted in a significant increase in bone mineral content and density, but a significant decrease in serum TRAP levels, urinary 8-hydroxydeoxyguanosine levels, and mRNA expression of tumor necrosis factor-a and cyclooxygenase-2 in spleen. A synergistic effect of GTP and alphacalcidol was observed in these parameters. Neither GTP nor alphacalcidol affected femoral bone area or serum osteocalcin. Conclusion: We conclude that a bone-protective role of GTP plus alphacalcidol during chronic10 inflammation bone loss may be due to a reduction of oxidative stress damage and inflammation.
C.-L. Shen , J. K. Yeh , O. L. Tatum , R. Y. Dagda , J.-S. Wang
1alpha-hydroxycholecalciferol , acid phosphatase , acid tolerance , bioavailability , bone density , bone resorption , dietary supplements , drinking water , epicatechin , epigallocatechin , females , gene expression , green tea , inflammation , lipopolysaccharides , messenger RNA , mineral content , osteocalcin , oxidative stress , polyphenols , prostaglandin synthase , rats , spleen , synergism , tumor necrosis factor-alpha , women's health
Osteoporosis International 2010 11 1 v.21 no.11
Journal Articles, USDA Authors, Peer-Reviewed
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