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Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli 0157 Isolates Associated with Human Infection

Permanent URL:
http://handle.nal.usda.gov/10113/58034
Abstract:
Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.
Author(s):
Indira T. Kudva , Sandra Smole , Robert W. Griffin , Jeonifer Garren , Nimisha Kalia , Megan Murray , Manohar John , Ralph Timperi , Stephen B. Calderwood
Subject(s):
Escherichia coli O157 , human diseases , polymerase chain reaction , pulsed-field gel electrophoresis
Source:
The Open Microbiology Journal 2013 v.7
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.