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Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism

Permanent URL:
http://handle.nal.usda.gov/10113/58026
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Abstract:
The origin of cultivated potatoes has remaining questions. In this study, 237 accessions of all cultivated species and 155 accessions of wild species closely related to cultivated potatoes, including their putative ancestors, were analyzed using 15 plastid microsatellites (SSRs) to investigate genetic diversity and their relationships with the wild species. We here used polymorphic plastid SSRs we developed from potato plastid genome sequences as well as already known plastid SSR markers. All 15 loci were polymorphic and identified a total of 127 haplotypes. Dramatic decreases in levels of genetic diversity were revealed in landraces in comparison with wild ancestor species. The plastid SSR results showed a decrease in haplotype number and diversity from Peru to both north and south. Phylogenetic analysis revealed two distinct groups. One of them, group A, contained the majority of accessions of cultivated species of the Solanum tuberosum Andigenum group including all accessions of cultivated diploid and triploid cytotypes of this group (S. chaucha, S. phureja, and S. stenotomum by a former taxonomic system) and most of tetraploid accessions of the S. tuberosum Andigenum group (S. tuberosum subsp. andigenum), and the majority of accessions of wild ancestors from the northern members of the S. brevicaule complex. Another group B comprised most of the wild species accessions and almost exclusively hybrid cultivated species which have introgressed plastid genomes from the other wild gene pools. Lack of clustering of traditional cultivated species (as used above) support a revised group classification of S. tuberosum.
Author(s):
Tatjana Gavrilenko , Olga Antonova , Anna Shuvalova , Ekaterina Krylova , Natalia Alpatyeva , David M. Spooner , Lubov Novikova
Subject(s):
Solanum brevicaule , Solanum chaucha , Solanum phureja , Solanum stenotomum , Solanum tuberosum , cytotypes , diploidy , genes , genetic markers , genetic polymorphism , genetic variation , haplotypes , hybrids , landraces , loci , microsatellite repeats , phylogeny , plastid DNA , potatoes , tetraploidy , triploidy , wild relatives , Peru
Source:
Genetic resources and crop evolution 2013 10 15 v.60 no.7
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.