Search National Agricultural Library Digital Collections

NALDC Record Details:

Identification of the Rice Blast Resistance Gene Pib in the National Small Grains Collection

Permanent URL:
http://handle.nal.usda.gov/10113/57985
File:
Download [PDF File]
Abstract:
The Pib gene in rice confers resistance to a wide range of races of the rice blast pathogen, Magnaporthe oryzae, including race IE1k that overcomes Pita, another broad-spectrum resistance gene. In this study,the presence of Pib was determined in 164 rice germplasm accessions from a core subset of the National Small Grains Collection utilizing DNA markers and pathogenicity assays. The presence of Pib was evaluated with two simple sequence repeat (SSR) markers and a dominant marker (Pib-dom) derived from the Pib gene sequence. Pathogenicity assays using two avirulent races (IE1k and IB1) and a virulent race (IB54) were performed to verify the resistance responses of accessions. Of the 164 accessions evaluated, 109 contained the Pib gene as determined using both SSR markers and pathogenicity assays, albeit different haplotypes were detected. The remaining 52 germplasm accessions were different in their responses to the blast races IB54, IE1k, and IB1, thus indicating the presence of R gene(s) other than Pib. The accessions characterized in this study could be used for marker-assisted breeding to improve blast resistance in indica and japonica cultivars worldwide.
Author(s):
M. RoyChowdhury , Y. Jia , M. H. Jia , R. Fjellstrom
Subject(s):
Magnaporthe oryzae , blast disease , cultivars , disease resistance , genes , genetic markers , germplasm , haplotypes , marker-assisted selection , microsatellite repeats , pathogenicity , races , rice
Source:
Phytopathology 2012 v.102 no.7
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.