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Influence of Primer Sequences and DNA Extraction Method on

Permanent URL:
http://handle.nal.usda.gov/10113/57938
Abstract:
Non-O157 Shiga toxin–producing Escherichia coli (STEC) infections, particularly those caused by the ‘‘big six’’ or ‘‘top six’’ non-O157 serogroups (O26, O45, O103, O111, O121, and O145) can result in severe illness and complications. Because of their significant public health impact and the notable prevalence of STEC in cattle, methods for detection of the big six non-O157 STEC in ground beef have been established. Currently, the U.S. Department of Agriculture, Food Safety and Inspection Service detection methods for screening beef samples for non-O157 STEC target the stx1, stx2, and eae virulence genes, with the 16S rRNA gene as an internal control, in a real-time PCR multiplex assay. Further, the serogroup is determined by PCR targeting genes in the E. coli O-antigen gene clusters of the big six non-O157 serogroups. The method that we previously reported was improved so that additional stx variants, stx1d, stx2e, and stx2g, are detected. Additionally, alignments of the primers targeting the eae gene were used to improve the detection assay so that eae subtypes that could potentially be of clinical significance would also be detected. Therefore, evaluation of alternative real-time PCR assay primers and probes for the stx and eae reactions was carried out in order to increase the stx and eae subtypes detected. Furthermore, a Tris-EDTA DNA extraction method was compared with a previously used procedure that was based on a commercially available reagent. The Tris-EDTA DNA extraction method significantly decreased the cycle threshold values for the stx assay (P , 0.0001) and eae assay (P , 0.0001), thereby increasing the ability to detect the targets. The use of different stx primers and probes increased the subtypes detected to include stx1d, stx2e, and stx2g, and sequence data showed that modification of the eae primer should allow the known eae subtypes to be detected.
Author(s):
Jamie L. Wasilenko , Pina M. Fratamico , Neelam Narang , Glenn E. Tillman , Scott Ladely , Mustafa Simmons , William C. Cray Jr.
Subject(s):
DNA , DNA primers , Food Safety and Inspection Service , Shiga toxin-producing Escherichia coli , bacterial contamination , food contamination , ground beef , multigene family , nucleotide sequences , polymerase chain reaction , ribosomal RNA , sampling , serotypes , virulence , United States
Source:
Journal of food protection 2012 v.75 no.11
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.