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Insertional mutagenesis using Tnt1 retrotransposon in potato

Permanent URL:
http://handle.nal.usda.gov/10113/57934
Abstract:
Potato is the third most important food crop in the world. However, genetics and genomics research of potato has lagged behind many major crop species due to its autotetraploidy and a highly heterogeneous genome. Insertional mutagenesis using T-DNA or transposable elements, which is available in several crops, has not been established in potato. Here we report a successful application of using the Tnt1 retrotransposon as an insertional mutagen in potato. The Tnt1 retrotransposon was introduced into a highly homozygous and self-compatible clone, 523-3, of a diploid wild potato species Solanum chacoense. Transposition of the Tnt1 elements introduced into 523-3 can be efficiently induced by tissue culture. Tnt1 preferentially inserted into genic regions in the potato genome and the insertions were stable during sexual reproduction, making Tnt1 an ideal mutagen in potato. Several distinct phenotypes associated with plant statue and leaf morphology were discovered in mutation screening from a total of 38 families derived from Tnt1-containing lines. We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523-3 clone can be expanded to the genome-wide level to potentially tag every gene in the potato genome.
Author(s):
Saowapa Duangpan , Wenli Zhang , Yufang Wu , Shelley H. Jansky , Jiming Jiang
Subject(s):
Solanum chacoense , diploidy , genes , genomics , insertional mutagenesis , leaves , mutagenicity , phenotype , potatoes , retrotransposons , screening , sexual reproduction , tissue culture , transfer DNA , transposition (genetics) , transposons
Source:
Plant physiology 2013 9 1 v.163 no.1
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.