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Microbial interactions associated with secondary cucumber fermentation

Permanent URL:
http://handle.nal.usda.gov/10113/57792
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Abstract:
Aims: To evaluate the interaction between selected yeasts and bacteria and associate their metabolic activity with secondary cucumber fermentation. Methods and Results: Selected yeast and bacteria, isolated from cucumber secondary fermentations, were inoculated as single and mixed cultures in a cucumber juice model system. Our results confirmed that during storage of fermented cucumbers and in the presence of oxygen, spoilage yeasts are able to grow and utilize the lactic and acetic acids present in the medium, which results in increased brine pH and the chemical reduction in the environment. These conditions favour opportunistic bacteria that continue the degradation of lactic acid. Lactobacillus buchneri, Clostridium bifermentans and Enterobacter cloacae were able to produce acetic, butyric and propionic acids, respectively, when inoculated in the experimental medium at pH 4-6. Yeast and bacteria interactions favoured the survival of Cl. bifermentans and E. cloacae at the acidic pH typical of fermented cucumbers (3-2), but only E. cloacae was able to produce a secondary product. Conclusions: The methodology used in this study confirmed that a complex microbiota is responsible for the changes observed during fermented cucumber secondary fermentation and that certain microbial interactions may be essential for the production of propionic and butyric acids. Significance and Impact of the Study: Understanding the dynamics of the development of secondary cucumber fermentation aids in the identification of strategies to prevent its occurrence and economic losses for the pickling industry.
Author(s):
W. Franco , I. M. Perez-Diaz
Subject(s):
Clostridium bifermentans , Enterobacter cloacae , Lactobacillus buchneri , acetic acids , acidity , bacteria , brining , butyric acid , chemical reduction , cucumbers , fermentation , fermented foods , food spoilage , food storage , juices , lactic acid , mixed culture , model food systems , oxygen , pH , pickles , propionic acid , spoilage , yeasts
Source:
Journal of Applied Microbiology 2012 v.114
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.