Search National Agricultural Library Digital Collections

NALDC Record Details:

Evaluation of Salmonella movement through the gut of the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Permanent URL:
http://handle.nal.usda.gov/10113/57313
File:
Download [PDF File]
Abstract:
Aims: The lesser mealworm, Alphitobius diaperinus is an important poultry pest prevalent during production that is capable of vectoring pathogens. This study was undertaken to determine the gut transit time of Salmonella for biosecurity risk analysis of pathogen dispersal into the environment. Methods: Adult and larval A. diaperinus were exposed to two concentrations of a fluorescently labeled Salmonella enterica for 15, 30, and 60 min time periods then externally disinfected to evaluate internal transfer of Salmonella. The insects were monitored every 30 min over 4 h and evacuated frass (feces) processed for the marker Salmonella. The minimum time monitored was 45min (15 exposure + 30 min time point), and the maximum was 5 h (60 exposure + 4 h time point). Results: Adults treated with 10(6) or 10(8) colony-forming units (cfu)/mL, which produced Salmonella positive frass within the 5 h experimental time, displayed a mean gut transit time of 144.4 min (range 90–270 min) and 186.3 min (range 120–300 min), respectively. Larvae treated with 10(6) or 10(8) cfu/mL displayed a mean gut transit time of 172.5 min (range 120–300 min) and 131.7 min (range 60–300 min), respectively. Significance and Impact of Study: Understanding the sources and contribution of reservoir populations of pathogens in poultry production operations is important for development of biosecurity measures to mitigate their transfer. A. diaperinus are prevalent in production operations and difficult to suppress. Management standards accept the reutilization of litter in which insects survive between flock rotations. Removing litter and spreading it onto nearby fields results in the inadvertent dispersal of beetles. Few studies demonstrating the specific bacterial dispersal capacities of these insects have been performed. This study determined that Salmonella acquired internally, commonly transits the gut, allowed the insect to disperse viable pathogenic bacteria within 2–3h.
Author(s):
Longyu Zheng , Tawni L. Crippen , Cynthia L. Sheffield , Toni L. Poole , Ziniu Yu , Jeffrey K. Tomberlin
Subject(s):
Alphitobius diaperinus , Salmonella enterica , adults , bacteria , biosecurity , digestive system , feces , flocks , frass , gastrointestinal transit , imagos , insect larvae , insect pests , insect vectors , pathogens , poultry , poultry production , risk analysis
Source:
Vector-borne and zoonotic diseases 2012 4 1 v.12 no.4
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.