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Proteus mirabilis interkingdom swarming signals attract blow flies

Permanent URL:
http://handle.nal.usda.gov/10113/57305
File:
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Abstract:
Flies transport specific bacteria with their larvae that provide a wider range of nutrients for those bacteria. Our hypothesis was that this symbiotic interaction may depend on interkingdom signaling. We obtained Proteus mirabilis from the salivary glands of the blow fly Lucilia sericata; this strain swarmed significantly and produced a strong odor that attracts blow flies. To identify the putative interkingdom signals for the bacterium and flies, we reasoned that as swarming is used by this bacterium to cover the food resource and requires bacterial signaling, the same bacterial signals used for swarming may be used to communicate with blow flies. Using transposon mutagenesis, we identified six novel genes for swarming (ureR, fis, hybG, zapB, fadE and PROSTU_03490), then, confirming our hypothesis, we discovered that fly attractants, lactic acid, phenol, NaOH, KOH and ammonia, restore swarming for cells with the swarming mutations. Hence, compounds produced by the bacterium that attract flies also are utilized for swarming. In addition, bacteria with the swarming mutation rfaL attracted fewer blow flies and reduced the number of eggs laid by the flies. Therefore, we have identified several interkingdom signals between P. mirabilis and blow flies.
Author(s):
Qun Ma , Alicia Fonseca , Wenqi Liu , Andrew T. Fields , Meaghan L. Pimsler , Aline F. Spindola , Aaron M. Tarone , Tawni L. Crippen , Jeffery K. Tomberlin , Thomas K. Wood
Subject(s):
Lucilia sericata , Proteus mirabilis , ammonia , bacteria , foods , genes , insect attractants , lactic acid , larvae , mutagenesis , nutrients , odors , oviposition , phenol , potassium hydroxide , salivary glands , sodium hydroxide
Source:
The ISME Journal: Multidisciplinary Journal of Microbial Ecology 2012 v.1
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.