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Simultaneous determination of 13 fluoroquinolone and 22 sulfonamide residues in milk by a dual-colorimetric enzyme-linked immunosorbent assay

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Enzyme-linked immunosorbent assays (ELISAs) usually focus on the detection of a single analyte or a single group of analytes, e.g., fluoroquinolones or sulfonamides. However, it is often necessary to simultaneously monitor the two classes of antimicrobial residues in different food matrices. In this paper, we describe a dual-colorimetric ELISA for simultaneous detection of 13 fluoroquinolone and 22 sulfonamide residues. The limit of detection for fluoroquinolones and sulfonamides was 2.4 ng/mL and 5.8 ng/mL, respectively. The developed immunoassay is suitable for high-throughput screening of these low-molecular weight contaminants. This is the first report where two different enzymes (alkaline phosphatase and horseradish peroxidase) were used in one immunoassay and together in a single well for simultaneous detection of multiple low-molecular weight chemical residues.
Wenxiao Jiang , Zhanhui Wang , Ross C. Beier , Haiyang Jiang , Yongning Wu , Jianzhong Shen
alkaline phosphatase , antibiotic residues , colorimetry , detection limit , enzyme-linked immunosorbent assay , fluoroquinolones , food contamination , food matrix , milk , monitoring , multiresidue analysis , peroxidase , screening , sulfonamides
Analytical Chemistry 2013 1 24 v.85
Journal Articles, USDA Authors, Peer-Reviewed
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