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Impact of by-product feedstuffs in Escherichia coli O157:H7 and Salmonella Typhimurium in Pure and Mixed Ruminal and Fecal Culture in Vitro

Permanent URL:
http://handle.nal.usda.gov/10113/57236
File:
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Abstract:
The use of by-product feedstuffs and prebiotics in animal diets has increased in recent years. The present study was undertaken to determine what effects novel by-product feedstuffs, including prebiotics, have on survival of the important foodborne pathogenic bacteria Escherichia coli O157:H7 and Salmonella enterica Typhimurium in pure and mixed ruminal and fecal culture fermentations from cattle and swine. Byproduct feedstuffs utilized in this study included: hyperimmunized whole egg, lysine biomass, lysine biomass (spray dried), threonine biomass (drum dried), threonine biomass (spray dried), beer well yeast (drum dried), beer well yeast (spray dried), ethanol yeast (pan dried) and corn meal as a control to simulate normal dietary conditions. Prebiotics examined included: PremiDex â„¢, CitriStimâ„¢, a CitriStim:PremiDex blend (50%:50%), and a commercial oligosaccharide source feedstuff. Pure culture populations of E. coli O157:H7 were reduced (P < 0.05) by 2% w/v of each of spray dried threonine, drum-dried threonine, ethanol yeast, hyper-immunized whole egg, and a blend of CitriStim:PremiDex. No effects on Salmonella populations were observed in pure cultures. Fermentations of mixed ruminal microorganisms from cattle fed a forage based diet demonstrated that 2% PremiDex reduced (P < 0.05) E. coli O157:H7 populations compared to controls i and the CitriStim:PremiDex blend reduced E. coli O157:H7 and Salmonella populations (P < 0.05) in fermentations from cattle fed high grain diets. The anti-foodborne pathogen effects appear to be an indirect effect mediated by the microbial population of the intestinal tract, such as has been reported previously for prebiotics.
Author(s):
T. R. Callaway , S. Block , R. C. Anderson , D. J. Nisbet
Subject(s):
Escherichia coli O157 , Salmonella typhimurium , beers , biomass , byproducts , cattle , cattle feeds , corn meal , eggs , feed grains , fermentation , forage , intestines , lysine , oligosaccharides , pathogens , prebiotics , rumen fermentation , rumen microorganisms , spray drying , swine , threonine , yeasts
Source:
Agriculture 2012 v.2
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.