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Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone etabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in animal tissues

Permanent URL:
http://handle.nal.usda.gov/10113/57231
File:
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Abstract:
A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) for determination of protein bound 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues is described to monitor the illegal use of furaltadone. Polyclonal and monoclonal antibodies were produced in this study. Rabbit polyclonal antibodies were used in the optimized cdELISA method and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC50 of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 µg/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The cdELISA method was validated by a previously established liquid chromatography–tandem mass spectrometry method. These results indicate that the cdELISA method developed here is a convenient, practical tool for screening large numbers of animal and fish tissue samples for the detection of AMOZ residues.
Author(s):
Peng Jie Luo , Wen Xiao Jiang , Ross C. Beier , Jian Zhong Shen , Hai Yang Jiang , Hong Miao , Yun Feng Zhao , Xia Chen , Yong Ning Wu
Subject(s):
animal tissues , cross reaction , detection limit , drug residues , enzyme-linked immunosorbent assay , fish , furaltadone , inhibitory concentration 50 , mass spectrometry , monitoring , monoclonal antibodies , polyclonal antibodies , protein binding , screening
Source:
Biomedical and Environmental Sciences 2012 8 28 v.25 no.4
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.