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Glycerol inhibition of ruminal lipolysis in vitro

Permanent URL:
http://handle.nal.usda.gov/10113/57221
Abstract:
Supplemental glycerol inhibits rumen lipolysis, a prerequisite for rumen biohydrogenation, which is responsible for the saturation of dietary fatty acids consumed by ruminant animals. Feeding excess glycerol, however, adversely affects dry matter digestibility. To more clearly define the effect of supplemental glycerol on rumen lipolysis, mixed populations of ruminal bacteria were incubated with 6 or 20% glycerol (vol/vol). After 48-h anaerobic incubation of mixed culture rumen fluid, rates of free fatty acid production (nmol/mL per h) for the 6 and 20% glycerol-supplemented samples were decreased by 80 and 86%, respectively, compared with rates from nonsupplemented control cultures (12.4 ± 1.0; mean ± SE). Conversely, assay of the prominent ruminal lipase-producing bacteria Anaerovibrio lipolyticus 5S, Butyrivibrio fibrisolvens 49, and Propionibacterium species avidum and acnes revealed no effect of 2 or 10% (vol/vol) added glycerol on lipolytic activity by these organisms. Supplementing glycerol at 6% on a vol/vol basis, equivalent to supplementing glycerol at approximately 8 to 15% of diet dry matter, effectively reduced lipolysis. However, the mechanism of glycerol inhibition of ruminal lipolysis remains to be demonstrated.
Author(s):
H. D. Edwards , R. C. Anderson , T. M. Taylor , M. D. Hardin , S. B. Smith , N. A. Krueger , D. J. Nisbet
Subject(s):
Anaerovibrio lipolyticus , Butyrivibrio fibrisolvens , Propionibacterium acnes , Propionibacterium avidum , biohydrogenation , diet , digestibility , fatty acids , free fatty acids , glycerol , lipolysis , mixed culture , rumen bacteria , rumen fluids , ruminants , triacylglycerol lipase
Source:
Journal of dairy science 2012 v.95
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.