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Bi-fluorescence imaging for estimating accurately the nuclear condition of Rhizoctonia spp.

Permanent URL:
http://handle.nal.usda.gov/10113/57105
Abstract:
Aims: To simplify the determination of the nuclear condition of the pathogenic Rhizoctonia, which currently needs to be performed either using two fluorescent dyes, thus more costly and time-consuming, or using only one fluorescent dye, thus less accurate. Methods and Results: A red primary fluorescence (autofluorescence) of the hyphal cell walls and septa of Rhizoctonia spp. with green excitation is evidenced in Rhizoctonia spp. This property is exploited and combined for the first time with a conventional DAPI fluorescence to accurately determine the nuclear condition of Rhizoctonia. This bi-fluorescence imaging strategy depicted the nuclear condition in Rhizoctonia spp. more accurately than the conventional DAPI fluorescence used alone and was validated against isolates previously genotyped by DNA sequencing. Conclusions: We demonstrated that the bi-fluorescence imaging strategy was safe, accurate and simple to perform and interpret. Significance and Impact of the Study: The developed bi-fluorescence imaging strategy provides a sensitive tool for determining the nuclear condition of Rhizoctonia strains. Its simplicity is a key advantage when there are numerous cultures to be examined.
Author(s):
F. Guermache , M. Rodier-Goud , A. Ceasar , C. Heraud
Subject(s):
Rhizoctonia , cell nucleus , cell walls , fluorescence , fluorescent dyes , hyphae , sequence analysis
Source:
Letters in Applied Microbiology 2012 v.54
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.