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Alpha-1 antitrypsin reduces ovariectomy-induced bone loss in mice

Permanent URL:
http://handle.nal.usda.gov/10113/56919
Abstract:
Proinflammatory cytokines are primary mediators of bone loss in estrogen deficiency. This study determined whether alpha-1 antitrypsin (AAT), a multifunctional protein with proteinase inhibitor and anti-inflammatory activities, mitigates bone loss induced by estrogen deficiency. Mice were either sham-operated or ovariectomized and injected with either AAT or phosphate buffered saline (PBS). Ovariectomy resulted in decreased wet uterus weight, significant bone loss, increased serum leptin concentrations, and higher body weight compared to sham. AAT injection increased tibial trabecular bone volume/total volume and trabecular thickness compared to PBS injection in ovariectomized mice. Ovariectomized mice with AAT treatment had higher uterus weight, lower serum osteocalcin levels, fewer bone marrow tartrate-resistant acid phosphatase-positive osteoclasts, and less expression of calcitonin receptor in bone than that in PBS-injected mice. These data demonstrate that AAT mitigates ovariectomy-induced bone loss in mice possibly through inhibiting osteoclast activity and bone resorption.
Author(s):
Jay J. Cao , Brian R. Gregoire , Li Sun , Sihong Song
Subject(s):
acid phosphatase , anti-inflammatory activity , blood serum , bone marrow , bone resorption , calcitonin , cytokines , estrogens , leptin , mice , osteocalcin , osteoclasts , ovariectomy , phosphates , receptors , tibia , tissue weight , trypsin inhibitors , uterus
Source:
Annals of the New York Academy of Sciences 2012 v.1240
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.