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Molecular Characterization and Gene Expression of the Channel Catfish Ferritin H Subunit After Bacterial Infection and Iron Treatment

Permanent URL:
http://handle.nal.usda.gov/10113/56892
Abstract:
Ferritins are the major iron storage protein in the cytoplasm of cells, responsible for regulating levels of intracellular iron. Ferritin genes are widely distributed in both prokaryotes and eukaryotes. In mammals, ferritin molecules are composed of heavy- (H) and light- (L) chain subunits; amphibian genomes contain three ferritin-type genes (H; middle, M; and L subunits); and teleost genomes to date contain H and M subunits. The objective of this study was to characterize the ferritin H gene in channel catfish (Ictalurus punctatus) to determine its genomic organization and copy numbers, to determine its patterns of tissue expression, and to establish if it is involved in defense responses of catfish after bacterial infection. The catfish ferritin H gene was completely sequenced and characterized, using both mRNA and genomic DNA. Catfish ferritin H gene has a full-length mRNA sequence of 999 bp, an open reading frame of 534 bp, and 4,704 bp genomic DNA sequence. Catfish ferritin H has a 5 exon and 4 intron genetic organization, containing a long 5'-untranslated region, which shares high similarity with mammalian and zebrafish genes. Based on phylogenetic analyses, the catfish ferritin H gene is highly conserved throughout evolution. Southern blot analysis suggested that the ferritin H gene has only one copy in the catfish genome. The catfish ferritin H gene was widely expressed in various healthy tissues. The catfish ferritin H gene was significantly up-regulated in the liver after intraperitoneal injection of iron dextran and coinjection of Edwardsiella ictaluri and iron dextran treatment, suggesting its role in iron metabolism and immunity.
Author(s):
Hong Liu , Tomokazu Takano , Eric Peatman , Jason Abernathy , Shaolin Wang , Zhenxia Sha , Huseyin Kucuktas , De-Hai Xu , Phillip Klesius , Zhanjiang Liu
Subject(s):
5' untranslated regions , DNA , Danio rerio , Edwardsiella ictaluri , Ictalurus punctatus , Southern blotting , amphibians , bacterial infections , catfish , cytoplasm , dextran , exons , ferritin , gene expression , genes , genomics , immunity , intraperitoneal injection , introns , iron , liver , mammals , metabolism , open reading frames , phylogeny , sequence analysis , storage proteins
Source:
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 2010 v.313 no.6
Language:
English
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.