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Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus

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Vibrio scophthalmi, a bacterial pathogen of olive flounder Paralichthys olivaceus, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of V. scophthalmi toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against V. scophthalmi infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD50 values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 ?g protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O2? overflow and intracellular O2? concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS.
Guo Qiao , In-Kwon Jang , Kyoung Mi Won , Sung Ho Woo , De-Hai Xu , Soo Il Park
Paralichthys olivaceus , Vibrio scophthalmi , adhesion , enzyme activity , fish , lethal dose 50 , leucine , macrophages , mucus , nitric oxide , oxygen , pathogens , resistance mechanisms , strains , superoxide dismutase , triacylglycerol lipase , virulence
Fisheries science 2013 v.79 no.1
Journal Articles, USDA Authors, Peer-Reviewed
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