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A highly sensitive and selective immunoassay for the detection of tetrabromobisphenol A in soil and sediment

Permanent URL:
http://handle.nal.usda.gov/10113/56462
File:
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Abstract:
Tetrabromobisphenol A (TBBPA) is the most widely used brominated flame retardant. A sensitive and selective enzyme-linked immunosorbent assay (ELISA) for the detection of TBBPA was developed. Six haptens (T1-T6) mimicking different structural elements of TBBPA were synthesized and coupled to keyhole limpet hemocyanin (KLH) or bovine serum albumin (BSA) as immunochemical reagents. The best ELISA was based on the combination of antiserum against KLH-T1 and coating antigen BSA-T2, showing an IC50 of 0.87 ng/mL and an IC10 (LOD) of 0.05 ng/mL for TBBPA in assay buffer, with high specificity. Concentrations of TBBPA determined by ELISA in the soils from farmlands, the soils from an e-waste recycling site, and the sediments of a canal ranged from non-detectable - 5.6 ng/g, 26 - 104 ng/g and 0.3 - 22 ng/g dw, respectively, indicating the ubiquitous pollution of this compound. The results of this assay for 16 real world samples displayed a good agreement with those of the LC-MS/MS method, indicating this ELISA is a suitable screening tool for TBBPA in environmental matrices.
Author(s):
Ting Xu , Jia Wang , Shang-zhong Liu , Cong Lu , Weilin L. Shelver , Qing X. Li , Ji Li
Subject(s):
agricultural land , agricultural soils , antiserum , bovine serum albumin , buffers , environmental fate , enzyme-linked immunosorbent assay , flame retardants , haptens , recycling , screening , sediments , soil pollution
Source:
Analytica Chimica Acta 2012 v.751
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.