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Genome size variation among sex types in dioecious and trioecious Caricaceae species

Permanent URL:
http://handle.nal.usda.gov/10113/56411
Abstract:
Caricaceae is a small family consisting of 35 species of varying sexual systems and includes economically important fruit crop, Carica papaya, and other species of ?highland papayas?. Flow cytometry was used to obtain genome sizes for 11 species in three genera of Caricaceae to determine if genome size differences can be detected between sexes. Genome sizes ranged from 442.5 to 625.9 megabases (Mb) likely due to variation in the accumulation of retrotransposons in the genomes. The C. papaya genome size was estimated to be 442.5 Mb, larger than previously reported. Significant differences were detected between male and female samples in Jacaratia spinosa, Vasconcellea horovitziana, and V. stipulata, and between male and hermaphrodite samples of V. cundinamarcensis, suggesting the presence of sex chromosomes for these species. The small size differences between genomes of the papaya sexes were not detected using flow cytometry. Vasconcellea horovitziana was discovered to have a larger female genome size than male, suggesting the possibility of a ZW sex chromosome system in the family. The estimated genome sizes of these 11 species will be used in sequencing their genomes and in sex chromosome research for this family.
Author(s):
Andrea R. Gschwend , Ching Man Wai , Francis Zee , Aru K. Arumuganathan , Ray Ming
Subject(s):
Carica papaya , Jacaratia spinosa , Vasconcellea , dioecy , females , flow cytometry , fruit crops , gender differences , genome , males , papayas , retrotransposons , sex chromosomes
Source:
Euphytica 2013 v.189 no.3
Language:
English
Year:
2013
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.