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Genetic characterization of North American populations of the wheat curl mite (Aceria tosichella) and dry bulb mite (Aceria tulipae)

Permanent URL:
http://handle.nal.usda.gov/10113/55925
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Abstract:
The wheat curl mite, Aceria tosichella Keifer, transmits at least three harmful viruses, wheat streak mosaic virus (WSMV), high plains virus (HPV), and Triticum mosaic virus (TriMV) to wheat (Triticum aestivum L.) throughout the Great Plains. This virus complex is considered to be the most serious disease of winter wheat in the western Great Plains. One component of managing this disease has been developing mite resistance in wheat; however, identification of mite biotypes has complicated deployment and stability of resistance. This biotypic variability in mites and differential virus transmission by different mite populations underscores the need to better understand mite identity. However, A. tosichella has a history of serious taxonomic confusion, especially as it relates to A. tulipae Keifer, the dry bulb mite. Molecular techniques were used to genetically characterize multiple A. tosichella populations and compare them to populations of A. tulipae. Mites from these populations were PCR amplified and the ribosomal ITS2 region sequenced. These results indicated limited variability between these two species, but two distinct types within A. tosichella were found that corresponded to previous work with Australian mite populations. Further work using sequencing of several mitochondrial DNA genes also demonstrated two distinct types of A. tosichella populations. Furthermore, the separation between these two A. tosichella types is comparable to their separation with A. tulipae, indicating that species scale differences exist between these two types of A. tosichella. These genetic differences correspond to important biological differences between the types (e.g. biotypic and virus transmission differences). In light of these differences, it is important that future studies on biological response differences account for these mite differences.
Author(s):
Gary L. Hein , Roy French , Benjawan Siriwetwiwat , James W. Amrine
Subject(s):
Aceria tosichella , Aceria tulipae , High Plains virus , Rhizoglyphus echinopus , Triticum aestivum , Wheat streak mosaic virus , biotypes , genes , internal transcribed spacers , mites , polymerase chain reaction , population genetics , species differences , virus transmission , viruses , winter wheat , Great Plains region
Source:
Journal of economic entomology 2012 v.105 no.5
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.