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NALDC Record Details:

Tritimovirus P1 functions as a suppressor of RNA silencing and an enhancer of disease symptoms

Permanent URL:
http://handle.nal.usda.gov/10113/55916
Abstract:
Wheat streak mosaic virus (WSMV) is an eriophyid mite-transmitted virus of the genus Tritimovirus, family Potyviridae. Complete deletion of HC-Pro has no effect on WSMV virulence or disease synergism, suggesting that a different viral protein suppresses post-transcriptional gene silencing (PTGS). PTGS suppression assays using Nicotiana benthamiana 16C plants expressing GFP were conducted with each WSMV protein; only P1 suppressed PTGS. Symptom enhancement assays were conducted by inoculating Potato virus X (PVX) onto transgenic N. benthamiana. Symptoms produced by PVX were more severe on transgenic plants expressing WSMV P1 or potyvirus HC-Pro compared to transgenic plants expressing GFP or WSMV HC-Pro.
Author(s):
Brock A. Young , Drake C. Stenger , Feng Qu , T. Jack Morris , Satyanarayana Tatineni , Roy French
Subject(s):
Nicotiana benthamiana , Potato virus X , RNA , Wheat streak mosaic virus , bioassays , gene silencing , proteinases , synergism , transgenic plants , viral proteins , virulence , viruses
Source:
Virus Research 2012 v.163 no.2
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.