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Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis

Permanent URL:
http://handle.nal.usda.gov/10113/55558
Abstract:
Yersinia ruckeri is the etiologic agent of enteric redmouth disease, a disease of salmonid fish species that is found worldwide in areas where salmonid fish species are farmed (3, 6, 18, 20). Vaccines for enteric redmouth disease have been used successfully for nearly 3 decades and consist of immersionapplied, killed whole-cell preparations of motile serovar 1 Y. ruckeri strains (22). Recently though, outbreaks have been reported in vaccinated fish at trout farms in the United Kingdom (2), Spain (9), and the United States (1). The Y. ruckeri strains isolated from these outbreaks are uniformly atypical serovar 1 isolates lacking both flagellar motility and secreted lipase activity. These variants have been classified as Y. ruckeri biotype 2 (BT2) and are believed to have a reduced sensitivity to immersion vaccination (2). The objective of this study was to obtain a better understanding of the emergence of BT2 Y. ruckeri by identifying genetic elements necessary for expression of the Y. ruckeri flagellum and determining the role that the flagellum plays in virulence by using a rainbow trout infection model.
Author(s):
Jason P. Evenhuis , Scott E. LaPatra , David W. Verner-Jeffreys , Inger Dalsgaard , Timothy J. Welch
Subject(s):
Oncorhynchus mykiss , Yersinia ruckeri , bacterial motility , biotypes , digestive system diseases , fish diseases , fish farms , flagellum , genes , models , mutagenesis , serotypes , transposons , triacylglycerol lipase , vaccination , vaccines , virulence , Spain , United Kingdom , United States
Source:
Applied and environmental microbiology 2009 Oct. v.75 no.20
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.