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Molecular responses of ceruloplasmin to Edwardsiella ictaluri infection and iron overload in channel catfish (Ictalurus punctatus)

Permanent URL:
http://handle.nal.usda.gov/10113/55527
Abstract:
Ceruloplasmin is a serum ferroxidase that carries more than 90% of the copper in plasma and has documented roles in iron homeostasis as well as antioxidative functions. In our previous studies, it has been shown that the ceruloplasmin gene is strongly up-regulated in catfish during challenge with Edwardsiella ictaluri. However, little is known about the function of this gene in teleost fish. The objective of this study, therefore, was to characterize the ceruloplasmin gene from channel catfish, determine its genomic organization, profile its patterns of tissue expression, and establish its potential for physiological antioxidant responses in catfish after bacterial infection with E. ictaluri and iron treatment. The genomic organization suggested that the catfish ceruloplasmin gene had 20 exons and 19 introns, encoding 1074 amino acids. Exon sizes of the catfish ceruloplasmin gene were close to or identical with mammalian and zebrafish homologs. Further phylogenetic analyses suggested that the genewas highly conserved through evolution. The catfish ceruloplasmin gene was mapped to both the catfish physical map and linkage map. The catfish ceruloplasmin genewas mainly expressed in liver with limited expression in other tissues, and it was significantly up-regulated in the liver after bacterial infection alone or after co-injection with bacteria and iron-dextran, while expression was not significantly induced with iron-dextran treatment alone.
Author(s):
Hong Liu , Eric Peatman , Wenqi Wang , Jason Abemathy , Shikai Liu , Huseyin Kucuktas , Jeffery Terhune , De-Hai Xu , Phillip Klesius , Zhanjiang Liu
Subject(s):
Edwardsiella ictaluri , Ictalurus punctatus , antioxidant activity , bacterial infections , catfish , chromosome mapping , copper , exons , ferroxidase , fish diseases , gene expression , gene expression regulation , genes , homeostasis , introns , iron , liver , phylogeny , physiological response
Source:
Fish and Shellfish Immunology 2011 v.30
Language:
English
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.