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Minimal influence of G‐protein null mutations on ozone‐induced changes in gene expression, foliar injury, gas exchange and peroxidase activity in Arabidopsis thaliana L.
- Abstract::
- Ozone (O3) uptake by plants leads to an increase in reactive oxygen species (ROS) in the intercellular space of leaves and induces signalling processes reported to involve the membrane‐bound heterotrimeric G‐protein complex. Therefore, potential G‐protein‐mediated response mechanisms to O3 were compared between Arabidopsis thaliana L. lines with null mutations in the α‐ and β‐subunits (gpa1‐4, agb1‐2 and gpa1‐4/agb1‐2) and Col‐0 wild‐type plants. Plants were treated with a range of O3 concentrations (5, 125, 175 and 300 nL L−1) for 1 and 2 d in controlled environment chambers. Transcript levels of GPA1, AGB1 and RGS1 transiently increased in Col‐0 exposed to 125 nL L−1 O3 compared with the 5 nL L−1 control treatment. However, silencing of α and β G‐protein genes resulted in little alteration of many processes associated with O3 injury, including the induction of ROS‐signalling genes, increased leaf tissue ion leakage, decreased net photosynthesis and stomatal conductance, and increased peroxidase activity, especially in the leaf apoplast. These results indicated that many responses to O3 stress at physiological levels were not detectably influenced by α and β G‐proteins.
- Author(s):
-
FITZGERALD BOOKER , KENT BURKEY , PATRICK MORGAN , EDWIN FISCUS , ALAN JONES
- Subject(s):
-
Arabidopsis thaliana , G-proteins , enzyme activity , extracellular space , gas exchange , gene expression , gene induction , genes , growth chambers , ions , leaves , mutation , ozone , peroxidase , photosynthesis , plant damage , reactive oxygen species , stomatal conductance , stress response , wild plants
- Source:
- Plant, cell & environment 2012 4 v.35 no.4
- Language:
- English
- Year:
- 2012
- Publisher:
- Blackwell Publishing Ltd
- Collection:
-
Journal Articles, USDA Authors, Peer-Reviewed
- File:
-
Download [PDF]
- Rights:
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
