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Short report: The effect of preservation methods on predicting mosquito age by near infrared spectroscopy

Permanent URL:
http://handle.nal.usda.gov/10113/54989
File:
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Abstract:
Determining mosquito age is important to evaluate vector control programs because the ability to transmit diseases is age dependent. Current age-grading techniques require dissection or RNA extraction. Near infrared spectroscopy has been used to rapidly and nondestructively determine the age of fresh mosquitoes and specimens stored in RNAlater, but other preservation techniques have not been examined. Thus, in this study, we investigate whether age can be predicted from insects preserved by various common methods. Results from this study show that age can be predicted from mosquitoes preserved with desiccants, ethanol, Carnoy, RNAlater, or refrigeration with confidence intervals < 1.4 days. The best results were generally obtained from mosquitoes stored using desiccants, RNAlater, or refrigeration.
Author(s):
Dowell, Floyd E. , Noutcha, Aline E.M. , Michel, Kristin
Subject(s):
Culicidae , RNA , age , age determination , confidence interval , desiccants , disease transmission , ethanol , insects , near-infrared spectroscopy , prediction , refrigeration , vector control
Note:
Includes references
Source:
The American journal of tropical medicine and hygiene: official journal of the American Society of Tropical Medicine and Hygiene. 2011 Dec., v. 85, no. 6
Language:
English
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.