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Pressurized liquid extraction and quantification of anthocyanins in purple-fleshed sweet potato genotypes

Permanent URL:
http://handle.nal.usda.gov/10113/54507
Abstract:
Analysis of anthocyanins responsible for the purple flesh color is important for breeding programs and development of value-added products. This study aimed to optimize the conditions for anthocyanin extraction from purple-fleshed sweet potatoes (PFSP) using pressurized-liquid extraction (PLE) method and quantify anthocyanins in various genotypes. Freeze-dried powders of PFSP genotypes were extracted with acidified methanol using an accelerated solvent extractor. Anthocyanin content of the extract was characterized by (a) pH-differential method and (b) color value protocol measuring absorbance at 530nm, which is commonly used in Japan as a commercial indicator of total anthocyanin quantity. Highest anthocyanin yields by PLE were with an acetic acid:methanol:water mixture of 7:75:18% (v/v), sample weight of <0.5g and 80–120°C. Among 335 genotypes, the anthocyanin content varied widely from 0 to 663mg cyanidine-3-glucoside equivalent/100g powder or 0–210mg/100g fresh weight. The total monomeric anthocyanin (TMA) contents determined by the pH-differential method were highly correlated with the Japan color value (JCV) protocol, TMA=(0.145) JCV, R2=0.943. These results can be useful for sweet potato breeding programs and processing industry in development of PFSP cultivars and processed products with high anthocyanin levels.
Author(s):
V.D. Truong , Z. Hu , R.L. Thompson , G.C. Yencho , K.V. Pecota
Subject(s):
anthocyanins , color , cultivars , extractors , food analysis , food processing , freeze drying , genotype , industry , methanol , plant breeding , powders , sweet potatoes , Japan
Source:
Journal of food composition and analysis 2012 May - June v.26 no.1-2
Language:
English
Publisher:
Elsevier Inc.
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.